||Recombinant Human GSTK1 protein was expressed inE.coliand purified by using conventional chromatography techniques, 25.5 kDa (226aa).
||GSTK1 is a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification process. This protein catalyzes the conjugation of the thiol group of glutathione (GSH) to the electrophilic groups of a wide range of hydrophobic substrates, leading to an easier removal of the latter from the cells.
|Sequences Of Amino Acids:
||MGPLPRTVEL FYDVLSPYSW LGFEILCRYQ NIWNINLQLR PSLITGIMKD SGNKPPGLLP RKGLYMANDL KLLRHHLQIP IHFPKDFLSV MLEKGSLSAM RFLTAVNLEH PEMLEKASRE LWMRVWSRNE DITEPQSILA AAEKAGMSAE QAQGLLEKIA TPKVKNQLKE TTEAACRYGA FGLPITVAHV DGQTHMLFGS DRMELLAHLL GEKWMGPIPP AVNARL.
||Liquid. In 20mM Tris buffer(pH 8.0) containing 10% glycerol 1mM DTT.
||> 90% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Drug metabolism - cytochrome P450; Glutathione metabolism; Metabolism of xenobiotics by cytochrome P450; Peroxisome