Recombinant Human HIPK1, GST-tagged HIPK1-516H

Recombinant Human HIPK1, GST-tagged


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Recombinant Human HIPK1, GST-tagged

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Cat. No. : HIPK1-516H
Product Overview : Recombinant human HIPK1 (156-555) was expressed inSf9cells using an N-terminal GST tag,71kDa.
Description : HIPK1 or homeodomain-interacting protein kinase 1 is a ser/thr protein kinase and a member of the HIPK family. HIPK 1 is a nuclear kinase that phosphorylates homeodomain transcription factors. HIPK1 phosphorylates DAXX and this leads to its relocalization and subsequent decrease in transcriptional repression activity. HIPK1also interacts with p53 and phosphorylates it on serine residues. HIPK 1 expression is elevated in breast cancer cell lines and embryonic fibroblasts from HIPK 1-null mice show more susceptibility to apoptosis induced by DNA damage .
Source : Sf9.
Formulation : Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Purity : The purity was determined to be >90% by densitometry. Camk2α Approx.
Concentration : 0.1 μg/μl.
Specific Activity : The specific activity of HIPK1 was determined to be 79 nmol /min/mg as per activity assay protocol.
Storage And Stability : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : HIPK1 homeodomain interacting protein kinase 1 [ Homo sapiens ]
Synonyms : homeodomain interacting protein kinase 1; Myak; Nbak2; KIAA0630; MGC26642; MGC33446; MGC33548; HIPK1; homeodomain-interacting protein kinase 1; nuclear body associated kinase 2b; homeodomain interacting protein kinase 1-like protein;OTTHUMP00000013763; OTTHUMP00000013768; EC
Gene ID : 204851
mRNA Refseq : NM_152696.3
Protein Refseq : NP_689909.2
MIM : 608003
UniProt ID : Q86Z02
Chromosome Location : 1p13.2
Function : protein serine/threonine kinase activity; ATP bindingnucleotide binding; protein binding; transferase activity

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