| Cat. No.: | Nucb2-1959R |
| Product Overview: | Recombinant Rat Nesfatin-1 (a.a. 25-106 of rat nesfatin-1) was expressed inE. coli,8 kDa. |
| Description: | Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein, expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behavior. Intracerebroventicular injection of nesfatin-1 reduces body weight in rats be affecting the animal food intake. |
| Source: | E. coli. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute with sterile water to a concentration of 0.1-1 mg/ml. Further dilutions should be made with medium containing 0.2% BSA. |
| Endotoxin: | <0.1 EU/μg purified protein (LAL test). |
| Purity: | >95% by SDS-PAGE. |
| Form: | Lyophilized from PBS. |
| Storage Conditions: | The lyophilized protein should be stored desiccated below 0°C. Stable for 6 months. Reconstituted Nesfatin-1 should be stored in working aliquots at -20°C. Avoid freeze/thaw cycles. |
| Gene Name: | Nucb2 nucleobindin 2 [ Rattus norvegicus ] |
| Synonyms: | nucleobindin 2; p54; Nefa; Nucb2; NEFA; nucleobindin-2; DNA-binding protein NEFA |
| Gene ID: | 59295 |
| mRNA Refseq: | NM_021663 |
| Protein Refseq: | NP_067695 |
| UniProt ID: | Q9JI85 |
| Chromosome Location: | 1q35 |
| Function: | DNA binding; calcium ion binding |
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