||Recombinant Rat Nesfatin-1 (a.a. 25-106 of rat nesfatin-1) was expressed inE. coli,8 kDa.
||Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein, expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behavior. Intracerebroventicular injection of nesfatin-1 reduces body weight in rats be affecting the animal food intake.
||Centrifuge the vial prior to opening. Reconstitute with sterile water to a concentration of 0.1-1 mg/ml. Further dilutions should be made with medium containing 0.2% BSA.
||<0.1 EU/μg purified protein (LAL test).
||>95% by SDS-PAGE.
||Lyophilized from PBS.
||The lyophilized protein should be stored desiccated below 0°C. Stable for 6 months. Reconstituted Nesfatin-1 should be stored in working aliquots at -20°C. Avoid freeze/thaw cycles.