Recombinant Human FK506 Binding Protein 2, 13kDa, His-tagged
Cat.No. : | FKBP2-2033H |
Product Overview : | Recombinant human FKBP2 protein was expressed in E.coli and purified by using conventional chromatography techniques. MW=13.4 kDa (122aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | FKBP2-2033H |
Description : | FKBP2 is peptidyl proline isomerase enzyme and a member of the immunophilin protein family. This protein is predominantly expressed in thymus and T cells and plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. FKBP2 is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. |
Source : | Escherichia Coli. |
Sequences Of Amino Acids : | MATGAEGKRK LQIGVKKRVD HCPIKSRKGD VLHMHYTGKL EDGTEFDSSL PQNQPFVFSL GTGQVIKGWD QGLLGMCEGE KRKLVIPSEL GYGERGAPPK IPGGATLVFE VELLKIERRT EL. |
Purity : | > 90% by SDS–PAGE. |
Concentration : | 1 mg/ml (determined by Bradford assay). |
Form : | Liquid. In 20mM Tris buffer(pH 8.0) containing 10% glycerol, 1mM DTT. |
Biological activity : | Specific activity is > 270 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin. |
Activity Assay : | 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant FKBP2 protein with 1ug in assay buffer.3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C. |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | FKBP2 FK506 binding protein 2, 13kDa [ Homo sapiens ] |
Synonyms : | FKBP2; FK506 binding protein 2, 13kDa; PPIase; FKBP-13; FKBP2; peptidyl-prolyl cis-trans isomerase FKBP2; FKBP-2; rotamase; 13 kDa FKBP; PPIase FKBP2; proline isomerase; immunophilin FKBP13; rapamycin-binding protein; 13 kDa FK506-binding protein; EC 5.2.1.8; FK506-binding protein 2; Rotamase; Immunophilin FKBP13; 13 kDa FK506-binding protein; Peptidyl-prolyl cis-trans isomerase; rapamycin-binding protein |
Gene ID : | 2286 |
mRNA Refseq : | NM_001135208 |
Protein Refseq : | NP_001128680 |
MIM : | 186946 |
UniProt ID : | P26885 |
Chromosome Location : | 11q13.1-q13.3 |
Function : | FK506 binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity; protein binding |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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