Recombinant Human TNF protein, FLAG-tagged

Cat.No. : TNF-412H
Product Overview : Recombinant Human TNF(aa 85-233) fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag was expressed in HEK293.
Source : HEK293
Species : Human
Tag : FLAG
Form : Lyophilized. Contains PBS.
Bio-activity : Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Molecular Mass : ~34kDa (SDS-PAGE)
Endotoxin : <0.02EU/μg purified protein (LAL test; Lonza).
Purity : ≥95% (SDS-PAGE)
Stability : Stable for at least 6 months after receipt when stored at -20 centigrade.
Storage : Short Term Storage: +4 centigrade;
Long Term Storage: -20 centigrade.
After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
Concentration : 0.1mg/ml after reconstitution.
Reconstitution : Reconstitute with 100μl sterile water.
Publication :
Gene Name : TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha;
Gene ID : 7124
mRNA Refseq : NM_000594
Protein Refseq : NP_000585
MIM : 191160
UniProt ID : P01375
Chromosome Location : 6p21.3
Pathway : Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem;
Function : cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding;

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