Recombinant Human TNF protein, FLAG-tagged

Cat.No.: TNF-412H
Product Overview: Recombinant Human TNF(aa 85-233) fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag was expressed in HEK293.
Source: HEK293
Species: Human
Tag : FLAG
Form: Lyophilized. Contains PBS.
Bio-activity: Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Molecular Mass: ~34kDa (SDS-PAGE)
Endotoxin: <0.02EU/μg purified protein (LAL test; Lonza).
Purity: ≥95% (SDS-PAGE)
Stability: Stable for at least 6 months after receipt when stored at -20 centigrade.
Storage: Short Term Storage: +4 centigrade;
Long Term Storage: -20 centigrade.
After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
Concentration: 0.1mg/ml after reconstitution.
Reconstitution: Reconstitute with 100μl sterile water.
Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
Official Symbol: TNF
Synonyms: TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha;
Gene ID: 7124
mRNA Refseq: NM_000594
Protein Refseq: NP_000585
MIM: 191160
UniProt ID: P01375
Chromosome Location: 6p21.3
Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem;
Function: cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding;

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