Active Recombinant Human TNF protein, FLAG-tagged
Cat.No. : | TNF-412H |
Product Overview : | Recombinant Human TNF(aa 85-233) fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | FLAG |
Form : | Lyophilized. Contains PBS. |
Bio-activity : | Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells). |
Molecular Mass : | ~34kDa (SDS-PAGE) |
Endotoxin : | <0.02EU/μg purified protein (LAL test; Lonza). |
Purity : | ≥95% (SDS-PAGE) |
Stability : | Stable for at least 6 months after receipt when stored at -20 centigrade. |
Storage : | Short Term Storage: +4 centigrade; Long Term Storage: -20 centigrade. After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
Concentration : | 0.1mg/ml after reconstitution. |
Reconstitution : | Reconstitute with 100μl sterile water. |
Publication : |
Effects of methylprednisolone on inflammatory activity and oxidative stress in the lungs of brain-dead rats. (2013)
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Gene Name : | TNF tumor necrosis factor [ Homo sapiens ] |
Official Symbol : | TNF |
Synonyms : | TNF; tumor necrosis factor; TNFA, tumor necrosis factor (TNF superfamily, member 2); DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; TNFA; TNF-alpha; |
Gene ID : | 7124 |
mRNA Refseq : | NM_000594 |
Protein Refseq : | NP_000585 |
MIM : | 191160 |
UniProt ID : | P01375 |
Chromosome Location : | 6p21.3 |
Pathway : | Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; |
Function : | cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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