||Recombinant Human Early Growth Response 4 is produced inSf9 Cells.
||The recombinant EGR-4 protein can be employed in the following fields of research: a) Promoter studies: Identification of EGR-4 binding promoter elements. b) Detection of EGR-4 interacting proteins. c) Signal transduction: Assay for the effect on the nuclear component EGR-4. d) Screening for EGR-4 activity in tumor cells and lines. e) Drug screening: Effect on EGR-4 generation and DNA binding.
||Liquid. In 20mM HEPES, pH 7.9, containing 25% glycerol, 420mM sodium chloride, 0.2mM EDTA and 1.5mM magnesium chloride.
||Binds specifically to an oligonucleotide with the sequence GCG GGG GCG.
||EMSA promoter characterization, in vitro transcription assay, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as positive control).
||-80°C. Avoid freeze/thaw cycles.