Recombinant Mouse Lymphocyte-Activation Gene 3, Fc Chimera

• Cat.No.: Lag3-2089M
• Product Overview: Recombinant MouseLag3 Produced inCHO cells.The sequence coding for the 4 extracellular Ig-like domains of mouse LAG-3 (D1-D4) is fused to the Fc portion of mouse IgG2a (hinge-CH2-CH3). MW=180kDa (SPS-PAGE, nonreducing), ~80kDa (SPS-PAGE, reducing).

Specification

• Cat. No.: Lag3-2089M
• Description: The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa).
• Source: CHO cells.
• Concentration: 0.5mg/ml.
• Purity: ≥95% (SDS-PAGE).
• Formulation: Liquid. In PBS.
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
• Specificity: Binds to human and mouse MHC class II.
• Biological Activity: Induces maturation of mouse dendritic cells in vitro. Induces CD4 and CD8 antigen-specific T cells responses when coinjected s.c. in mice, together with the antigen.
• Application: Flow Cytometry.
• Long Trem Storage: +4°C.
• Stability: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at+4°C.

Gene Information

• Gene Name: Lag3 lymphocyte-activation gene 3 [ Mus musculus ]
• Synonyms: Lag3; lymphocyte-activation gene 3; Ly66; CD223; LAG-3
• Gene ID: 16768
• mRNA Refseq: NM_008479
• Protein Refseq: NP_032505
• UniProt ID: Q61790
• Chromosome Location: 6 F2
• Function: MHC class II protein binding; transmembrane receptor activity