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Recombinant Human Protein Kinase, cGMP-dependent, Type I

Cat. No.: PRKG1-604H
Product Overview: Recombinant Human PRKG1 was produced inSf9 cells.MW = 76kDa; monomer.
Description: PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain. By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation. Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3.
Source: Sf9 cells.
Purity: ≥95% (SDS-PAGE).
Formulation: Liquid. In 20mM TRIS, pH 7.4, containing 1mM EDTA, 1mM β-mercaptoethanol, 100mM NaCl, 10U/ml trasylol and 50% glycerol.
Concentration: 50µg/ml.
Specific Activity: (1)Basal activity: 0.1U/mg protein. 22-fold stimulation by cGMP (5µM). (2)Max. activity: 2.2U/mg. (3)One unit corresponds to 1µmol/mg/min using VASPtide (RRKVSKQE) as substrate under the following optimal conditions: 10mM HEPES, pH 7.4, containing 5mM MgCl2, 1mM DTE, 0.2mM EDTA, 30℃.
Storage: Enzyme is relatively unstable. To prevent loss of activity when performing experiments, keep the stock vial of the enzyme on ice at all times. Enzyme loses activity during a single freeze/thaw cycle. After opening, prepare aliquots and store at -20°C.
Gene Name: PRKG1 protein kinase, cGMP-dependent, type I [ Homo sapiens ]
Synonyms: PRKG1; protein kinase, cGMP-dependent, type I; PGK; PKG; CGKI; PRKG1B; PRKGR1B; FLJ36117; MGC71944; cGKI-BETA; cGKI-alpha; DKFZp686K042; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I; EC 2.7.11.12
Gene ID: 5592
mRNA Refseq: NM_001098512
Protein Refseq: NP_001091982
MIM: 176894
UniProt ID: Q13976
Chromosome Location: 10q11.2
Pathway: Gap junction; Long-term depression; Olfactory transduction; Vascular smooth muscle contraction
Function: ATP binding; cGMP binding; cGMP-dependent protein kinase activity; nucleotide binding; protein serine/threonine kinase activity; transferase activity

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