|Cat. No. :
|Product Overview :
||Recombinant Human PRKG1 was produced inSf9 cells.MW = 76kDa; monomer.
||PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain. By Northern blot analysis PRKG1 was shown to be expressed at highest levels in bladder, uterus, adrenal gland, and fallopian tube. PRKG1 plays an important stimulatory role in platelet activation. Expression of recombinant PRKG1 in a reconstituted cell model enhanced von Willebrand factor-induced activation of the platelet integrin alpha-IIb/beta-3.
||Liquid. In 20mM TRIS, pH 7.4, containing 1mM EDTA, 1mM β-mercaptoethanol, 100mM NaCl, 10U/ml trasylol and 50% glycerol.
|Specific Activity :
||(1)Basal activity: 0.1U/mg protein. 22-fold stimulation by cGMP (5µM). (2)Max. activity: 2.2U/mg. (3)One unit corresponds to 1µmol/mg/min using VASPtide (RRKVSKQE) as substrate under the following optimal conditions: 10mM HEPES, pH 7.4, containing 5mM MgCl2, 1mM DTE, 0.2mM EDTA, 30℃.
||Enzyme is relatively unstable. To prevent loss of activity when performing experiments, keep the stock vial of the enzyme on ice at all times. Enzyme loses activity during a single freeze/thaw cycle. After opening, prepare aliquots and store at -20°C.