Recombinant HCV Polyprotein, His-tagged

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Recombinant HCV Polyprotein, His-tagged

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Cat. No. : HCVgp1-2052H
Product Overview : Recombinant HCV NS3 protein fused to His-tag was expressed inE.coliand purified by using conventional chromatography techniques. 27.9 kDa (268aa).
Description : HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation.
Source : E.coli.
Form : Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
Purity : > 95% by SDS - PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : HCVgp1 polyprotein [ Hepatitis C virus ]
Synonyms : Polyprotein; HCVgp1; core protein; E1 protein; E2 protein; p7 protein; NS2 protein; NS3 protease/helicase; NS4A protein; NS4B protein; NS5A protein; NS5B RNA-dependent RNA polymerase
Gene ID : 951475
Protein Refseq : NP_671491

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