||Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. 41.5 kDa (384aa).
||Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia.
||> 95% by SDS- PAGE.
||In 20mM Tris-HCl buffer (pH8.0) containing 100mM NaCl,10% glycerol.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MPYQYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR QLLLTADDRV NPCI GGVILF HETLYQ KADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPSALAIMENANV LARYASICQQ N GIV PIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HIYLEGTLLK PNMVTPGHAC TQKFSHEEIAMATVTALRRT VPPAVTGITF LSGGQSEEEA SIN LNAINKC PLLKPWALTF SYGRALQASA LKAWGGKKEN LKAAQEEYVK RALA NSLACQ GKYTPSGQAG AAASESLFVS NHAY.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.