||Recombinant Aprataxin protein fused to his-tag was expressed inE.coliand purified by using conventional chromatography techniques. 23.9kDa (205aa).
||Aprataxin is a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. Aprataxin may play a role in single-stranded DNA repair. Mutations in the gene have been associated with neurological disorder known as ataxia oculomotor apraxia-1.
||> 95% by SDS – PAGE.
||Liquid. 20mM Tris-HCl buffer (pH7.5) containing 20% glycerol,100mM NaCl, 0.1mM PMSF.
||0.25 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMQD PKM QVY KDEQ VVVIKDKYPK ARYHWLVLPW TSISSLKAVA REHLELLKHMHTVGEKVIVD FAGSSKLRFR LGYHAIPSMS HVHLHVISQD FDSPCLKNKK HWNSFNTEYF LE SQAVIEMV QEAGRVTVRD GMPELLKLPLRCHECQQ LLP SIPQ LKEHL R KH WT Q.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.