Recombinant Human Aprataxin, His-tagged

• Cat.No.: APTX-1935H
• Product Overview: Recombinant Aprataxin protein fused to his-tag was expressed inE.coliand purified by using conventional chromatography techniques. 23.9kDa (205aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Aprataxin is a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. Aprataxin may play a role in single-stranded DNA repair. Mutations in the gene have been associated with neurological disorder known as ataxia oculomotor apraxia-1.
• Purity: > 95% by SDS – PAGE.
• Physical Appearance: Liquid.
• Formulation: Liquid. 20mM Tris-HCl buffer (pH7.5) containing 20% glycerol,100mM NaCl, 0.1mM PMSF.
• Concentration: 0.25 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMQD PKM QVY KDEQ VVVIKDKYPK ARYHWLVLPW TSISSLKAVA REHLELLKHMHTVGEKVIVD FAGSSKLRFR LGYHAIPSMS HVHLHVISQD FDSPCLKNKK HWNSFNTEYF LE SQAVIEMV QEAGRVTVRD GMPELLKLPLRCHECQQ LLP SIPQ LKEHL R KH WT Q.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: APTX aprataxin [ Homo sapiens ]
• Synonyms: APTX; aprataxin; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT; MGC1072; FLJ20157; forkhead-associated domain histidine triad-like protein; ataxia 1, early onset with hypoalbuminemia
• Gene ID: 54840
• mRNA Refseq: NM_017692
• Protein Refseq: NP_060162
• MIM: 606350
• UniProt ID: Q7Z2E3
• Chromosome Location: 9p13.3
• Function: DNA 5"-adenosine monophosphate hydrolase activity;chromatin binding;damaged DNA binding;double-stranded DNA binding;double-stranded RNA binding;hydrolase activity;metal ion binding;phosphoglycolate phosphatase activity;protein N-terminus binding;protein binding;zinc ion binding