Recombinant Human CHI3L1 protein, His-tagged
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|Product Overview :||Recombinant Human CHI3L1 (Tyr22-Thr383) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Chitinase-3-like protein 1 (CHI3L1) is also known as Cartilage glycoprotein 39 (CGP-39), YKL-40, which belongs to the glycosyl hydrolase 18 family. CHI3L1 is present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. CHI3L1 may play a role in defense against pathogens, or in tissue remodeling and also may play an important role in the capacity of cells to respond to and cope with changes in their environment.|
|Predicted N Terminal :||Tyr22|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 41.0 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Tyr22-Thr383|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of CHI3L1-657H :||
Human CHI3L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||CHI3L1|
|Official Symbol :||CHI3L1|
|Synonyms :||CHI3L1; chitinase 3-like 1 (cartilage glycoprotein-39); chitinase-3-like protein 1; GP39; YKL40; 39 kDa synovial protein; cartilage glycoprotein 39; ASRT7; GP-39; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39; FLJ38139; DKFZp686N19119|
|Gene ID :||1116|
|mRNA Refseq :||NM_001276|
|Protein Refseq :||NP_001267|
|UniProt ID :||P36222|
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