Active Recombinant Human CPM protein, His-tagged

• Cat.No.: CPM-660H
• Product Overview: Recombinant Human CPM protein(NP_938079.1)(Met1-His422), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to release Larginine from BenzoylAlaArg, with detection of the arginine amino group by ophthaldialdehyde. The specific activity is >40,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human CPM comprises 416 amino acids and has a predicted molecular mass of 47.7 kDa as estimated in SDS-PAGE under reducing conditions.
• Protein Length: Met1-His422
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CPM carboxypeptidase M [ Homo sapiens ]
• Official Symbol: CPM
• Synonyms: CPM; carboxypeptidase M; renal carboxypeptidase; urinary carboxypeptidase B
• Gene ID: 1368
• mRNA Refseq: NM_001874
• Protein Refseq: NP_001865
• MIM: 114860
• UniProt ID: P14384