Recombinant Human Hemochromatosis Type 2 (Juvenile), FLAG-tagged

• Cat.No.: HFE2-2419H
• Product Overview: Recombinant Human RGMC (aa 36-398) produced in HEK 293 cells is fused at the N-terminus to a linker peptide (8 aa) and a FLAG-tag.

Specification

• Cat. No.: HFE2-2419H
• Description: The RGMC (also called Hemojuvelin) is an GPI-anchored protein involved in the iron metabolism through regulation of hepcidin expression. Mutations in the gene encoding RGMC cause severe iron overload (juvenile hemochromatosis /hemochromatosis type 2A). The protein binds to neogenin and acts as co-receptor of the bone morphogenetic protein. The mature RGMc protein is proteolytically processed and glycosylated.
• Source/Host: HEK 293 cells.
• Concentration: 0.1mg/ml after reconstitution.
• Purity: ≥95% (SDS-PAGE).
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
• Short Term Storage: -20°C.
• Formulation: Lyophilized. Contains PBS.
• Long Term Storage: -20°C.
• Use/Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Handling: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: HFE2 hemochromatosis type 2 (juvenile) [ Homo sapiens ]
• Synonyms: hemochromatosis type 2 (juvenile); JH; HJV; RGMC; HFE2A; MGC23953; HFE2; hemojuvelin; haemojuvelin; RGM domain family member C; hemochromatosis type 2 protein; Hemochromatosis type 2 protein; RGM domain family, member C; LEAP1; HAMP; HEPC
• Gene ID: 148738
• mRNA Refseq: NM_145277
• Protein Refseq: NP_660320
• MIM: 608374
• UniProt ID: Q6ZVN8
• Chromosome Location: 1q21.1