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Recombinant Mouse Tnfsf12, FLAG-tagged

Cat. No.: Tnfsf12-196M
Product Overview: Recombinant Mouse Tnfsf12 isproduced inHEK 293 cells.The extracellular domain of mouse TWEAK (aa 94-249) is fused at the N-terminus to tenascin-C (TNC) (chicken) (aa 110-139) and a FLAG-tag.
Description: Some TNF ligands (such as TWEAK (mouse) or FasL (mouse)) produced as soluble forms do not assemble into stable trimers and show low receptor binding. Addition of the tenascin-C oligomerization domain (TNC) (from chicken) at the N-terminus of recombinant mouse soluble TWEAK stabilizes the TNF homology domain (THD) of rmsTWEAK into a trimer and increases binding to its respective TNF receptor.
Source: HEK 293 cells.
Purity: ≥95% (SDS-PAGE).
Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
Concentration: 0.1mg/ml after reconstitution.
Formulation: Lyophilized. Contains PBS.
Reconstitution: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Stability: Stable for at least 6 months after receipt when stored at -20°C.
Storage: Long term storage: -20°C, short term storage: +4°C. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Gene Name: Tnfsf12 tumor necrosis factor (ligand) superfamily, member 12 [ Mus musculus ]
Synonyms:
Tnfsf12; tumor necrosis factor (ligand) superfamily, member 12; DR3L; APO3L; DR3LG; Tweak; tumor necrosis factor ligand superfamily member 12; OTTMUSP00000006216; TNF-related weak inducer of apoptosis
Gene ID: 21944
mRNA Refseq: NM_011614
Protein Refseq: NP_035744
UniProt ID: O54907
Chromosome Location: 11
Pathway: Cytokine-cytokine receptor interaction
Function: cytokine activity; tumor necrosis factor receptor binding

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