||Recombinant Humanunc-5 homolog A (C. elegans) produced inCHO cells.The extracellular domain of UNC5A (UNC5H1) (aa 26-306) is fused to the Fc portion of human IgG1. MW=80kDa.
||The receptors of the UNC family (for uncoordinated) include UNC-5 of C. elegans and its four vertebrate homologs, UNC5A, B, C and D (previously called UNC5H1-4). The UNC5 family binds to the netrin family of ligands and mediates axon repulsion effect of netrin, either alone or by binding to the DCC (another netrin receptor). These receptors are type I transmembrane proteins of roughly 110-120kDa composed of two extracellular Ig-like domains, two thrombospondin type I domains, and intracellular sequence with a ZU5 domain and a death domain. UNC5B is a direct target gene for p53 that mediates p53-dependent apoptosis, and is also a dependence receptor that regulates apoptosis either positively or negatively depending on its binding with netrin-1.
||1mg/ml after reconstitution.
||<0.1EU/µg purified protein (LAL test; Bio Whittaker).
||Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
||After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
|Storage And Stability:
||Stable for at least 6 months after receipt when stored at -20°C.