||Recombinant human macrophage inflammatory protein-1β was expressed inS. frugiperdainsect cells. A low molecular weight chemokine involved in inflammation, hematopoiesis, and immunoregulation. Exhibits chemoattractant activity on monocytes and chemoattractant and proadhesive effects on lymphocytes. MW = 7800.
||Macrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now also named CCL3 and CCL4. Both factors are produced by macrophages after they are stimulated with bacterial endotoxins. MIP-1a and MIP-1b activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. MIP-1a and MIP-1b induce synthesis and release of other pro-inflammatory cytokines such as interleukin-1 (IL-1), IL-6 and TNF-alpha from fibroblasts and macrophages. CCL3 and CCL4 genes are both located on human chromosome 17.
||≥97% by SDS-PAGE.
||Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg MIP-1β.
||ED50 = 40-60 ng/ml as measured by its ability to inhibit mouse hematopoietic stem cell proliferation in an in vitro colony assay that detects primitive cells.
||≤100 pg/µg MIP-1β.
||Sterile PBS containing ≥0.1% HSA or BSA.