||Recombinant human checkpoint homologue kinase 2 (Chk2) fused to a His Tag sequence at the N-terminus was expressed inS. frugiperdainsect cells. MW = 65430 Da.
||Chk2 is involved in sensing DNA damage in eukaryotic cells. It plays an important role in the control of the cell cycle by blocking replication.
||Liquid. In 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5. Please refer to vial label for lot-specific concentration.
||≥ 25,000 units/mg protein.
||≥ 95% by SDS PAGE.
||One unit is defined as the amount of enzyme required to transfer 1 picomol phosphate to the synthetic peptide substrate KKKVSRSGLYRSPSMPENLNRPR per min at 30°C.
||Standard Handling (MSDS available upon request).
||≤ -70 °C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C).