||Recombinant Human Cell Division Cycle 25 Homolog C (S. Pombe) is expressed inE.coli.
||Cdc25 is a dual-specificity phosphatase first isolated from the yeast Schizosaccharomyces pombe as a cell cycle defective mutant. As with other cell cycle proteins such as Cdc2 and Cdc4, the "cdc" in its name refers to "cell division cycle". Dual-specificity phosphatases are considered a sub-class of protein tyrosine phosphatases. By removing inhibitory phosphate residues from target Cyclin-Dependent Kinases (Cdks, Cdc25 proteins control entry into and progression through various phases of the cell cycle, including mitosis and S ("Synthesis") phase.
||Greater than 90 % pure as determined by SDS-PAGE analysis.
||48 kDa bandby SDS-PAGE analysis.
||The recombinant Cdc25C (catalytic domain) is supplied frozen in a buffer containing 20mM Tris-HCl (pH 8.2), 1mM EDTA, 2 mM DTT and 50% glycerol.
||Stable for 12 months at -70°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot enzyme to avoid repeated freezing and thawing.