Recombinant Human Apolipoprotein E, E2 Isoforms

• Cat.No.: APOE-2630H
• Product Overview: Recombinant human apolipoprotein E-E2 (cysteine at amino acids 112 and 158) expressed in S.frugiperda insect cellsusing a baculovirus expression system. Binds to β-amyloid protein but not to the LDL receptor. Does not compete with human low density lipoprotein for binding to the human Apo B/E (LDL) receptor.

Specification

• Cat. No.: APOE-2630H
• Description: ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution.
• Source: S. frugiperda insect cells.
• Form: Liquid.
• Formulation: In 700 mM NH4HCO3.
• Molar Mass: 34000Da.
• Purity: ≥95% by SDS-PAGE.
• Storage: ≤ -70oC. Ship: Dry Ice Only.
• Publication:
  Imipramine and olanzapine block apoE4-catalyzed polymerization of Aβ and show evidence of improving Alzheimer’s disease cognition (2021)
  The Effects of L-Cysteine on Alzheimer’s Disease Pathology in APOE2, APOE3, and APOE4 Homozygous Mice (2016)

Gene Information

• Gene Name: APOE apolipoprotein E [ Homo sapiens ]
• Synonyms:
  APOE; apolipoprotein E; AD2; LPG; LDLCQ5; MGC1571; apolipoprotein E3; Apo-E
• Gene ID: 348
• mRNA Refseq: NM_000041
• Protein Refseq: NP_000032
• MIM: 107741
• UniProt ID: P02649
• Chromosome Location: 19q13.31
• Pathway: Alzheimer"s disease; Metabolism of lipids and lipoproteins
• Function: antioxidant activity; beta-amyloid binding; cholesterol transporter activity; lipid transporter activity; low-density lipoprotein receptor binding; phosphatidylcholine-sterol O-acyltransferase activator activity; protein heterodimerization activity; very-low-density lipoprotein receptor binding