2x Taq PCR Master Mix

• Cat.No.: CMC07

Specification

• Tag: Non
• Description: 2× Taq PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. The 2× Taq PCR Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2-4kb.
• PCR Master Mix, 2×: 50 units/ml of Taq DNA Polymerase supplied in a proprietary reaction buffer (pH 8.5), 400μM each of dATP, dGTP, dCTP, dTTP, 3mM MgCl2.
• Feature: Fast: Set up reactions in less than a minute. Sensitive: Amplify as little as 2 copies of target template. High Fidelity: 2× Taq PCR Master Mix exhibits lower error rate than normal Taq DNA polymerase. Convenient: One tube, one pipetting step. Complete: Reagents, including Taq DNA Polymerase, MgCl2, dNTPs and buffers, in one tube. Scalable: Set up 10μl, 25μl or 50μl reactions. Stable: Stable for hundreds freeze-thaw cycles.
• Performance Guarantee: Creative BioMart PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Creative BioMart PCR product, we will send a replacement or refund your account.
• Applications: PCR. 3´ A-tailing of blunt ends, compatible with T-Vectors.
• Quality Control Tests: PCR, activity, Bacterial DNA contamination, endo- and exo-nuclease.
• Storage: Store at -20°C.
• Reaction Conditions: To amplify a 1kb DNA template Set up PCR reactions as follows: 2× Taq PCR Master Mix 25μl 10μM Primers 1μl each Template ~1μl ddH2O 22μl