|Cat. No. :
||2× Pfu PCR Master Mix is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. The 2× Pfu PCR Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2-3kb.
|PCR Master Mix, 2× :
||50 units/ml of Pfu DNA Polymerase supplied in a proprietary reaction buffer (pH 8.5), 400μM each of dATP, dGTP, dCTP, dTTP, 4mM MgSO4.
||Fast: Set up reactions in less than a minute. Sensitive: Amplify as little as 2 copies of target template. High Fidelity: 2x Pfu PCR Master Mix exhibits the lowest error rate of any thermostable DNA polymerase. Convenient: One tube, one pipetting step. Complete: Reagents, including Pfu DNA Polymerase, MgSO4, dNTPs and buffers, in one tube. Scalable: Set up 10μl, 25μl or 50μl reactions. Stable: Stable for hundreds freeze-thaw cycles.
|Performance Guarantee :
||Creative BioMart PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Creative BioMart PCR product, we will send a replacement or refund your account.
||2× Pfu PCR Master Mix is recommended for use in PCR, primer extension reactions and other applications that demand high fidelity.
|Quality Control Tests :
||PCR, activity, endo- and exo- nuclease.
||Store at -20°C.
|Reaction Conditions: :
||To amplify a 1kb DNA template Set up PCR reactions as follows: 2× Pfu PCR Master Mix 25μl 10μM Primers 1μl each Template ~1μl ddH2O 22μl