|Cat. No. :
|Product Overview :
||Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 31.4 kDa (284aa).
||HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit.
||Liquid. In 20 mM Tris-HCl Buffer (pH 8.5) containing 10% Glycerol.
||> 95% by SDS - PAGE.
||0.5 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids :
||MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDP VQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKLVKLESGLKVY GGDD RIG ALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAG CGKFYE GTADEMCKALLEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGN AAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDP VTTMR AVRREKDQFK MPRD.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.