"Hotstart Taq DNA polymerase" Related Products

Hotstart Taq DNA polymerase

Cat. No.: CMC15
Description: HotStart Taq DNA Polymerase is a thermostable, modified form of recombinant Taq DNA Polymerase. It is inactive at temperatures below 75°C, but can be activated by a 4 min 95°C incubation step. The combination of HotStart Taq DNA Polymerase and the optimized PCR buffer minimizes non-specific amplification products and primer dimers allowing highest sensitivity.
Formulation: HotStart Taq DNA polymerase, 10x HotStart reaction buffer(without Mg2+), 25mM MgCl2
Application: Amplification of genomic DNA and cDNA targets up to 3 kb long with high specificity, sensitivity, and yield, Real time PCR, Multiplex PCR, Carry-over prevention, Automated PCR e.g. handling at room temperatures.
Example: Useλ-DNA as template to do PCR. PCR condition:94℃ 5min/30cycles 94℃ 30sec, 58℃ 30sec, 72℃ 45sec/72℃ 5min.
Storage: -20℃

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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