||Recombinant human PTPRF was expressed inE.Coli.
||PTPRF or LAR is a member of the protein tyrosine phosphatase family with an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains. PTPRF has been shown to function in the regulation of epithelial cell-cell contacts at adherents junctions as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance.
||LAR, a leukocyte antigen related protein, is a transmembrane receptor-like protein tyrosine phosphatase (PTPase). Its cytoplasmic domain comprises two tandem domains possessing PTPase homology. Only one domain, LAR-D1, has phosphatase activity.
||LAR-D1 catalytic domain comprises amino acids 1275-1613.
||Solution containing 10 mM imidazole, pH 7.0, 2 mM EDTA, 5 mM DTT, 0.1 M NaCl, and 50% glycerol.
||Buffered aqueous glycerol solution.