||Recombinant human AUH protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography.MW = 31.4 kDa (293aa).
||AUH, also known as mitochondrial methylglutaconyl-CoA hydratase, is involved in the amino acid degradation pathway by catalyzing the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and water. It has been found to have very low enoyl-CoA hydratase activity.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
||> 95% by SDS - PAGE.
||1.0 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MSSEMKTEDE LRVRHLEEEN RGIVVLGINR AYGKNSLSKN LIKMLSKAVD ALKSDKKVRT IIIRSEVPGI FCAGADLKER AKMSSSEVGP FVSKIRAVIN DIANLPVPTI AAIDGLALGG GLELALACDI RVAASSAKMG LVETKLAIIP GGGGTQRLPR AIGMSLAKEL IFSARVLDGK EAKAVGLISH VLEQNQEGDA AYRKALDLAR EFLPQGPVAM RVAKLAINQG MEVDLVTGLA IEEACYAQTI PTKDRLEGLL AFKEKRPPRY KGE.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Metabolic pathways; Valine, leucine and isoleucine degradation; Metabolism of amino acids and derivativesV