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Recombinant Human CD8a Molecule, His-tagged, active

Cat.No. : CD8A-3196H
Product Overview : Recombinant Human CD8A Protein (rhCD8A) Ser 22 - Asp 182 (Accession # AAH25715), fused with 6×His tag at the C-terminus, was produced in human 293 cells (HEK293).
Description : Cluster of Differentiation 8 alpha chain (CD8A), also known as Cluster of Differentiation 8a (CD8A), is a single-pass type I membrane protein. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8A has been shown to interact with CD3D,[1] HLA-G[2-3] and HLA-A.[2, 4] The homodimer CD8alpha(alpha) or the heterodimer alpha beta stabilizes the interaction of the T-cell antigen receptor (TCR) with major histocompatibility complex (MHC) class I/peptide by binding to the class I molecule.[4] Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Ser 22
Form : Bulk protein in a 0.2um filtered solution of PBS, pH7.4 with 5% trehalose as protectant and delivered as lypohilized powder.
Molecular Mass : rhCD8A has a calculated MW of 18.4 kDa. DTT-reduced Protein migrates as 35 kDa due to glycosylation.
Endotoxin : Less than 1.0 EU per μg of the rhCD8A by the LAL method.
Purity : >85% as determined by SDS-PAGE.
Storage : Upon receipt, the product should be stored at -20℃ or lower for long term storage. Upon reconstitution, working aliquots should be stored at -70℃. Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: 4℃ for 1 year in lyophilized state after receipt; -70℃ for 3 months under sterile conditions after reconstitution with carrier protein.
Reconstitution : Before opening, centrifuge each vial for 20-30 seconds at 10,000g RCF to recover protein powder to the bottom of the vial. It is recommended to reconstitute the lyophilized rhCD8A in 1000ul sterile PBS, pH7.4 to a final concentration of 50 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. Please kindly note that this reconstitution instruction above MUST be followed to ensure successful reconstitution, and any improper handling will lead to loss or destroy of the activity. Avoid vigorous shaking or vortexing.
Publication :
Gene Name : CD8A CD8a molecule [ Homo sapiens ]
Official Symbol : CD8A
Synonyms : CD8A; CD8a molecule; CD8, CD8 antigen, alpha polypeptide (p32) , T cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; p32; Leu2;
Gene ID : 925
mRNA Refseq : NM_001145873
Protein Refseq : NP_001139345
MIM : 186910
UniProt ID : P01732
Chromosome Location : 2p12
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem;
Function : MHC class I protein binding; coreceptor activity; protein binding; protein homodimerization activity; protein kinase binding;

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