Active Recombinant Human CR2 protein(Met1-Arg971), His-tagged

• Cat.No.: CR2-3186H
• Product Overview: Recombinant Human CD21 isoform A (P20023-1) extracellular domain (Met 1-Arg 971) was expressed in HEK293, with a carboxy-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Arg971
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD21 His at 2 μg/ml (100 μl/well) can bind Human Annexin VI/ANXA6 His, Biotinylated, the EC50 of Human Annexin VI/ANXA6 His, Biotinylated is 250-900 ng/mL.
• Molecular Mass: The secreted recombinant human CD21 pro form consists of 962 amino acids with the predicted molecular mass of 106 kDa. As a result of glycosylation, the apparent molecular mass of rhCD21 is approximately 110-120 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CR2 complement component (3d/Epstein Barr virus) receptor 2 [ Homo sapiens ]
• Official Symbol: CR2
• Synonyms: CR2; complement component (3d/Epstein Barr virus) receptor 2; complement receptor type 2; CD21; EBV receptor; complement C3d receptor; epstein-Barr virus receptor; CR; C3DR; SLEB9
• Gene ID: 1380
• mRNA Refseq: NM_001006658
• Protein Refseq: NP_001006659
• MIM: 120650
• UniProt ID: P20023