||Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 26.7 kDa (241aa).
||PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway.
||Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol 5mM DTT, 0.2M NaCl.
||> 90% by SDS - PAGE.
||0.25 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Proteasome; Apoptosis; Cell Cycle Checkpoints; Cell Cycle, Mitotic; DNA Replication; HIV Infection; Metabolism of amino acids and derivatives; Signaling by Wnt