Recombinant Human EPHA7 protein, His-tagged EPHA7-3228H

Recombinant Human EPHA7 protein, His-tagged

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Recombinant Human EPHA7 protein, His-tagged

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Cat.No. : EPHA7-3228H
Product Overview : Recombinant Human EPHA7 (Gln27-Ile556) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Ephrin type-A receptor 7 (EPHA7) is also known as EPH homology kinase 3 (EHK3), EPH-like kinase 11 (HEK11), which belongs to the protein kinase superfamily or tyr protein kinase family or ephrin receptor subfamily. EPHA7 / EHK3 contains 1 Eph LBD (Eph ligand-binding) domain, 2 fibronectin type-III domains, 1 protein kinase domain, 1 SAM (sterile alpha motif) domain. EHK-3/EPHA7 is widely expressed. The catalytic activity of EPHA7/EHK3 is "ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate". EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gln27
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 60 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln27-Ile556
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >98% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of EPHA7-3228H :

Human EphA7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Gene Name : EPHA7
Official Symbol : EPHA7
Synonyms : EPHA7; EPH receptor A7; EphA7; ephrin type-A receptor 7; Hek11; EK11; EHK-3; EPH-like kinase 11; EPH homology kinase 3; Eph homology kinase-3; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3; EHK3; HEK11
Gene ID : 2045
mRNA Refseq : NM_004440
Protein Refseq : NP_004431
MIM : 602190
UniProt ID : Q15375

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