"rnhA" Related Products

Recombinant E.coli rnhA

Cat. No. : RnhA-739E
Product Overview : Recombinant E.coli RNase H (RNaseHI) was over-expressed inE. coliand highly purified. MW is 17.6 kDa. MW = 17.6 kDa.
Description : Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact.
Source : E.coli.
Purity : Greater than 95% protein determined by SDS-PAGE.
Form : 50 units/ul in 20mM Tris-HCl (pH 7.5), 100mM KCl, 1mM DTT, 50% Glycerol.
Specific Activity : 100,000 units/mg protein.1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37℃.
Applications : 1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA .2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT). 3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA.
Reagents Supplied With Enzyme : 100 mM Tris-HCl (pH 8.0), 100 mM MgCl2, 500 mM NaCl, 10 mM DTT, 500 ug/ml BSA (Bovine Serum Albumin).
Caution : To avoide contamination of trace amounts of nucleic acids in BSA, use reaction buffer that does not contain BSA and use RNaseH at higher concentrations.
Storage : at -20℃.
Crystallographic structure of E. coli RNase HI. :

Gene Name : rnhA ribonuclease HI, degrades RNA of DNA-RNA hybrids [ Escherichia coli str. K-12 substr. MG1655 ]
Synonyms : rnhA; ribonuclease HI, degrades RNA of DNA-RNA hybrids; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin; EC; RNase HI, degrades RNA of DNA-RNA hybrids, participates in DNA replication
Gene ID : 946955
Protein Refseq : NP_414750
Pathway : DNA replication
Function : DNA-dependent DNA replication; A catabolic process; proteolysis

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