Recombinant E.coli rnhA

• Cat.No.: RnhA-739E
• Product Overview: Recombinant E.coli RNase H (RNaseHI) was over-expressed inE. coliand highly purified. MW is 17.6 kDa. MW = 17.6 kDa.

Specification

• Cat. No.: RnhA-739E
• Description: Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact.
• Source: E.coli.
• Purity: Greater than 95% protein determined by SDS-PAGE.
• Form: 50 units/ul in 20mM Tris-HCl (pH 7.5), 100mM KCl, 1mM DTT, 50% Glycerol.
• Specific Activity: 100,000 units/mg protein.1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37℃.
• Applications: 1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA .2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT). 3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA.
• Reagents Supplied With Enzyme: 100 mM Tris-HCl (pH 8.0), 100 mM MgCl2, 500 mM NaCl, 10 mM DTT, 500 ug/ml BSA (Bovine Serum Albumin).
• Caution: To avoide contamination of trace amounts of nucleic acids in BSA, use reaction buffer that does not contain BSA and use RNaseH at higher concentrations.
• Storage: at -20℃.

Gene Information

• Gene Name: rnhA ribonuclease HI, degrades RNA of DNA-RNA hybrids [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: rnhA; ribonuclease HI, degrades RNA of DNA-RNA hybrids; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin; EC 3.1.26.4; RNase HI, degrades RNA of DNA-RNA hybrids, participates in DNA replication
• Gene ID: 946955
• Protein Refseq: NP_414750
• Pathway: DNA replication
• Function: DNA-dependent DNA replication; A catabolic process; proteolysis