Species : |
E.coli |
Source : |
E.coli |
Tag : |
Non |
Description : |
Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. |
Purity : |
Greater than 95% protein determined by SDS-PAGE. |
Form : |
50 units/ul in 20mM Tris-HCl (pH 7.5), 100mM KCl, 1mM DTT, 50% Glycerol. |
Specific Activity : |
100,000 units/mg protein.1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37℃. |
Applications : |
1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA .2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT). 3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA. |
Reagents Supplied With Enzyme : |
100 mM Tris-HCl (pH 8.0), 100 mM MgCl2, 500 mM NaCl, 10 mM DTT, 500 ug/ml BSA (Bovine Serum Albumin). |
Caution : |
To avoide contamination of trace amounts of nucleic acids in BSA, use reaction buffer that does not contain BSA and use RNaseH at higher concentrations. |
Storage : |
at -20℃. |