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Recombinant Human CTGF, His-tagged

Cat.No. : CTGF-2605H
Product Overview : Recombinant Human Connective Tissue Growth Factor with His tag is expressed in HEK293 cell.
Description : Connective Tissue Growth Factor belongs to the CCN family of proteins. The CCN family presently consists of six members in human also known as: Cyr61 (Cystein rich 61), CTGF (Connective Tissue Growth Factor), Nov (Nephroblastoma Overexpressed gene), WISP-1, 2 and 3 (Wnt-1 Induced Secreted Proteins). The CCN genes encode secreted proteins associated with the Extracellular Matrix (ECM) and cell membrane. CCN proteins are matricellular proteins which are involved in the regulation of various cellular functions including: proliferation, differentiation, survival, adhesion and migration.
Source : HEK293
Species : Human
Tag : His
Form : Filtered (0.4 μm) and lyophilized in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4.
Molecular Mass : 36 kDa
Protein length : 329aa
AA Sequence : QNCSGPCRCP DEPAPRCPAG VSLVLDGCGC CRVCAKQLGE LCTERDPCDP HKGLFCHFGS PANRKIGVCT AKDGAPCIFG GTVYRSGESF QSSCKYQCTC LDGAVGCMPL CSMDVRLPSP DCPFPRRVKL PGKCCEEWVC DEPKDQTVVG PALAAYRLED TFGPDPTMIR ANCLVQTTEW SACSKTCGMG ISTRVTNDNA SCRLEKQSRL CMVRPCEADL EENIKKGKKC IRTPKISKPI KFELSGCTSM KTYRAKFCGV CTDGRCCTPH RTTTLPVEFK CPDGEVMKKN MMFIKTCACH YNCPGDNDIF ESLYYRKMYG DMAHHHHHH
Endotoxin : <1.0 EU/µg
Purity : Purity as determined by densitometric image analysis: >95%.
Applications : Cell culture and/or animal studies, ELISA, Western blotting.
Quality Control Test : BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein. LAL TEST to determine quantity of the endotoxin.
Storage : Store the lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Reconstitution : Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Gene Name : CTGF connective tissue growth factor [ Homo sapiens ]
Official Symbol : CTGF
Synonyms : CTGF; connective tissue growth factor; CCN2; IGFBP8; IBP-8; IGFBP-8; CCN family member 2; IGF-binding protein 8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; NOV2; HCS24; MGC102839;
Gene ID : 1490
mRNA Refseq : NM_001901
Protein Refseq : NP_001892
MIM : 121009
UniProt ID : P29279
Chromosome Location : 6q23.2
Pathway : Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem; Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem;
Function : fibronectin binding; growth factor activity; heparin binding; insulin-like growth factor binding;

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