||Recombinant human PNPO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 25.9 kDa (226aa) confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher).
||PNPO, also known as pyridoxine-5"-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MDPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP
||> 90% by SDS – PAGE.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl, 0.1mM PMSF.
||0.5 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.