|Cat. No. :
|Product Overview :
||Recombinant human HSD17B14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||HSD17B14, also known as 17-beta-hydroxysteroid dehydrogenase 14, belongs to the 17-beta-HSD family of proteins, which regulate the availability of steroids within various tissues rough out the body. It exists as a homotetramer that localizes to the cytoplasm and is highly expressed in brain, placenta, liver and kidney.
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATG TRYAGKVVVV TGGGRGIGAG IVRAFVNSGA RVVICDKDES GGRALEQELP GAVFILCDVT QEDDVKTLVS ETIRRFGRLD CVVNNAGHHP PPQRPEETSA QGFRQLLELN LLGTYTLTKL ALPYLRKSQG NVINISSLVG AIGQAQAVPY VATKGAVTAM TKALALDESP YGVRVNCISP GNIWTPLWEE LAALMPDPRA TIREGMLAQP LGRMGQPAEV GAAAVFLASE ANFCTGIELL VTGGAELGYG CKASRSTPVD APDIPS.
||> 95% by SDS – PAGE.
||32.4 kDa (306aa) confirmed by MALDI-TOF.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
||1mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.