||Recombinant human EIF1AX protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =18.6kDa (164aa), confirmed by MALDI-TOF.
||EIF1AX is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5" end of capped RNA. Itseems to be required for maximal rate of protein biosynthesis. And it enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA (I) to 40 S ribosomal subunits.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKAD VILKYNADEA RSLKAYGELP EHAKINETDT FGPGDDDEIQ FDDIGDDDED IDDI
||> 90% by SDS – PAGE.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 5mM DTT, 200mM NaCl.
||1mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.