||Recombinant human SCG5 protein (27-212 aa), fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||SCG5, also known as Neuroendocrine protein 7B2, is widely distributed in neuroendocrine tissues. It functions as a chaperone protein for the proprotein convertase PC2 and is required for the production of an active PC2 enzyme.
||MYSPRTPDRV SEADIQRLLH GVMEQLGIAR PRVEYPAHQA MNLVGPQSIE GGAHEGLQHL GPFGNIPNIV AELTGDNIPK DFSEDQGYPD PPNPCPVGKT ADDGCLENTP DTAEFSREFQ LHQHLSDPEH DYPGLGKWNK KLLYEKMKGG ERRKRRSVNP YLQGQRLDNV VAKKSVPHFS DEDKDPELEH HHHHH.
||Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl, 1mM DTT, 20% glycerol, 0.1mM PMSF.
||22.0 kDa (195aa),confirmed by MALDI-TOF.
||> 85% by SDS – PAGE.
||0.5 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.