Recombinant Human RAB Interacting Factor, His-tagged

• Cat.No.: RABIF-2799H
• Product Overview: Recombinant human RABIF protein (1-123 aa), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: RABIF-2799H
• Description: RABIF, also known as guanine nucleotide exchange factor MSS4, is a guanine-nucleotide-releasing protein that may participate in vesicular transport. This protein stimulates GTP-GDP exchange in Sec4 and Rab and binds to a subset of genetically related Rab proteins.
• Source: E.coli.
• Sequences: MGSSHHHHHH SSGLVPRGSH MEPAEQPSELVSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV SHE.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl, 1mM DTT, 20% glycerol.
• Molecular Weight: 16.0 kDa (143aa),confirmed by MALDI-TOF.
• Purity: > 85% by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RABIF RAB interacting factor [ Homo sapiens ]
• Synonyms:
  RABIF; RAB interacting factor; Ras-specific guanine-releasing factor 3; guanine nucleotide exchange factor MSS4; mammalian suppressor of SEC4; rab-interacting factor; MSS4; RASGFR3; RASGRF3
• Gene ID: 5877
• mRNA Refseq: NM_002871
• Protein Refseq: NP_002862
• MIM: 603417
• UniProt ID: P47224
• Chromosome Location: 1q32.1
• Function: guanyl-nucleotide exchange factor activity; metal ion binding; zinc ion binding