||Recombinant Human CSF3 was produced in Pseudomonas fluorescens. MW = 18.8 kDa.
||G-CSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is produced extracellularly by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, GCSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.
||> 97%, by SDS-CGE under reducing conditions.
||< 1.0 EU per 1 μg of the protein by the LAL method.
||Measured in a cell proliferation assay using NFS-60 mouse myeloblast cells. The ED50 for this effect is typically 10-60 pg/ml.
||Lyophilized in acetate buffer, pH 4.0, containing sucrose and glycine.
||Store at 2-8°C upon receipt, preferably desiccated. Reconstitute aseptically in sterile 20 mM acetic acid, pH 4.0, to a G-CSF concentration of 0.15-0.25 mg/mL. Store reconstituted G-CSF at -20°C (not in a frost-free freezer). Avoid repeated freeze-thaw.