| Description : |
The protein is a putative hematopoetic growth factor for neutrophils, and wase used as a treatment for cyclic hematopoesis in humans and dogs. |
| Source : |
E.coli |
| Species : |
Rat |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in 5 mM Sodium Citrate, pH 4.0. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg. |
| Molecular Mass : |
Approximately 21.5 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids. |
| Protein length : |
195 |
| AA Sequence : |
KKIPLLTVSSLPPSLPLPRSFLLKS LEQVRKIQARNTELLEQLCATYKLC HPEELVLFGHSLGIPKASLSSCSSQ ALQQTKCLSQLHSGLFLYQGLLQAL AGISSELAPTLDMLHLDVDNFATTI WQQMESLGVAPTVQPTQSTMPIFTS AFQRRAGGVLVTSYLQSFLETAHHA LHHLPRPAQKHFPESLFISI |
| Endotoxin : |
Less than 0.1 EU/μg of rRtG-CSF as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
