||Recombinant human RBP5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 18.1kDa (155aa), confirmed by MALDI-TOF.
||RBP5 belongs to the Retinol-binding proteins family. Assessment of retinol-binding protein is used to determine visceral protein mass in nutritional studies related to health. RBP5 is higher expression in adult kidney and liver and to a lesser extent in adult and fetal spleen, adult lymph nodes and appendix, and fetal liver and kidney. It is strongly decreased in hepatocellular carcinoma tissues.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MPPNLTGYYR FVSQKNMEDY LQALNISLAV RKIALLLKPD KEIEHQGNHM TVRTLSTFRN YTVQFDVGVE FEEDLRSVDG RKCQTIVTWE EEHLVCVQKG EVPNRGWRHW LEGEMLYLEL TARDAVCEQV FRKVR
||> 95% by SDS – PAGE.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl.
||0.5mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.