||Recombinant Rat DNA polymeraseβoverproduced inE. coliwith high enzymatic activity without any tag attached. The enzyme has molecular mass of 38 kDa. The amino acid sequence of the rat enzyme has 86% identity to the human homolog.
||DNA polymeraseβ is a distributive polymerase involved in base excision repair which repairs damaged DNA by excising modified bases (oxidized, methylated, deaminated etc.).
||Over 95% pure by SDS-PAGE analysis.
||1.3 mg/ml in 50mM Tris-HCl pH7.6, 0.3M KCl, 0.1mM EDTA, 1mM DTT, 20% Glycerol.
||1) For the studies on the mechanisms of base-excision repair of DNA damage. 2) As a positive control of western blotting with anti-DNA polymerase β antibody.
||90 unit/ul (1 unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37℃ in 60 min.)
||-20℃ (long period, 70℃).