||Recombinant Human EIF2AK1, full length, amino acids M1-G630, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, was expressed inSf9insect cells.MW = 100,546 Da.
||The HRI gene is localized to 7p22 where its 3" end slightly overlaps the 3" end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme.
||Baculovirus infected Sf9 cells.
||HRI was confirmed as HRI by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
||50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.030 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!