||Recombinant Human PKN1, Amino acids M1-C942, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells.MW = 136,910 Da.
||The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||PRK1 was confirmed as PRK1 by specific Western Blotting using anti PRK1 antibody.
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.164 μg/μl (Bradford method using BSA [Sigma, cat#A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!