Recombinant Human Cyclin-dependent Kinase 16, GST-His

• Cat.No.: CDK16-710H
• Product Overview: Recombinant Human CDK16, Amino acids R107-F496, mutationally activated: S153A, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9 cells.MW = 74,943 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 107-496 a.a.
• Description: The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: PCTAIRE1 was confirmed as PCTAIRE1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.731 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 1 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: CDK16 cyclin-dependent kinase 16 [ Homo sapiens ]
• Synonyms: CDK16; cyclin-dependent kinase 16; PCTK1; PCTAIRE; FLJ16665; PCTAIRE1; PCTGAIRE; cell division protein kinase 16; PCTAIRE-motif protein kinase 1; serine/threonine-protein kinase PCTAIRE-1; EC 2.7.11.22; PCTAIRE protein kinase 1
• Gene ID: 5127
• mRNA Refseq: NM_001170460
• Protein Refseq: NP_001163931
• MIM: 311550
• UniProt ID: Q00536
• Chromosome Location: Xp11.3-p11.23
• Function: ATP binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity