||Recombinant Human CDK16, Amino acids R107-F496, mutationally activated: S153A, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9 cells.MW = 74,943 Da.
||The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||PCTAIRE1 was confirmed as PCTAIRE1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.731 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!