||Recombinant Human TSSK2, Amino acids M1-T358, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 73,916 Da.
||TSSK2 is an intronless serine-threonine kinase that was originally detected on mouse chromosome 16. TSSK2 and TSSK1 share 72% overall amino acid identity and 83% identity in the kinase domain. Real-time PCR showed abundant TSSK2 expression in testis and lower levels in heart, brain, and placenta while Western blot analysis detected TSSK2 at the predicted molecular mass of 40kDa in human sperm and testis.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||TSSK2 was confirmed as human TSSK2 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
||0.378 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!