||RecombinantHuman PAK3, full length, amino acids M1-R544, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9insect cells. MW = 90,588 Da.
||PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||PAK3 was confirmed as PAK3 by by mass spectroscopy LC-ESI-MS/MS (Protagen AG,Germany).
||50 mM HEPES-NaOH, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.360 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!