||Recombinant Human PAK6, full length, amino acids M1-C681, N-terminally fused to GST-HIS6-Thrombin cleavage site, expressed inSf9insect cells. MW = 104,780 Da.
||PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||PAK6 was confirmed as PAK6 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
||50 mM HEPES-NaOH, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.082 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!