||Recombinant Human CK1-alpha1 Amino acids M1-F365, N-terminally fused to GST-HIS6-Thrombin cleavage site was expressed in Sf9 cells. MW = 71,434 Da.
||Casein kinase 1, alpha 1 has been shown to interact with Centaurin, alpha 1 and AXIN1.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||CK1-alpha1 was confirmed as human CK1-alpha1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.058 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!