||Recombinant Human EPHA1, Amino acids R569-D976, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 79,631 Da.
||EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||EPHA1 was confirmed as human EPHA1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
||0.071 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!