Recombinant Human EPH Receptor A1, GST-His EPHA1-743H

Recombinant Human EPH Receptor A1, GST-His

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Recombinant Human EPH Receptor A1, GST-His

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Cat. No. : EPHA1-743H
Product Overview : Recombinant Human EPHA1, Amino acids R569-D976, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 79,631 Da.
Description : EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
Source : Baculovirus infected Sf9 cells.
Purification : One-step affinity purification using GSH-agarose.
Product Identity : EPHA1 was confirmed as human EPHA1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
Storage Buffer : 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
Concentration : 0.071 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
Specific Activity : 4 pmol/μg×min.
Storage : -80°C. Avoid repeated freeze-thaw cycles!
PDB rendering based on 1x5a. :

Gene Name : EPHA1 EPH receptor A1 [ Homo sapiens ]
Synonyms : EPHA1; EPH receptor A1; EPH; EPHT; EPHT1; MGC163163; EphA1; EC 2.7.10.1; Tyrosine-protein kinase receptor EPH; eph tyrosine kinase 1; ephrin receptor EphA1; ephrin type-A receptor 1; erythropoietin-producing hepatoma amplified sequence; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2
Gene ID : 2041
mRNA Refseq : NM_005232
Protein Refseq : NP_005223
MIM : 179610
UniProt ID : P21709
Chromosome Location : 7q32-q36
Pathway : Axon guidance
Function : ephrin receptor activity; nucleotide binding; protein binding; receptor activity; transferase activity

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